For those who have told me it’s just not possible to get reliable qPCR in a home/DIY setting, I admit there’s some truth to this. It can be finicky and I’ve found lots of ways to make mistakes. But… https://twitter.com/RickByers/status/1251640286880698372

Here’s the results of a dilution series I did using human B2M primers on my own nasal sample - showing 82% efficiency with simple dye PCR and 91% efficiency with TaqMan probes.